TABLE 1.
Purification of xylose reductase from the cell extract of C. parapsilosis
| Procedure | Total protein (mg) | Total activity (U) | Specific activity (U/mg of protein) | Yield (%) | Purification (fold) |
|---|---|---|---|---|---|
| Cell extraction | 425 | 369 | 0.87 | 100 | 1.00 |
| Ammonium sulfate fractionation | 121 | 266 | 2.20 | 72.1 | 2.53 |
| DEAE-cellulose ion exchange chromatography | 28.2 | 90.2 | 3.20 | 24.4 | 3.68 |
| Sephadex G-100 gel filtration chromatography | 3.36 | 51.7 | 15.4 | 14.0 | 17.7 |
| Cibacron Blue 3GA affinity chromatography | 1.25 | 40.1 | 32.1 | 10.9 | 36.9 |
| Preparative electrophoresis | 0.79 | 32.9 | 41.7 | 8.9 | 47.9 |