TABLE 3.
Properties of aldose reductases from various organisms
| Organism (source or reference) | Mr, subunit (kDa) | Mr, native (kDa) | Coenzyme | pI | Km (Xylose) (mM) | kcat/Km (s−1 mM−1) | Km (NAPDH) (μM) | Km (NADH) (μm) | Optimal pH |
|---|---|---|---|---|---|---|---|---|---|
| C. parapsilosis (this work) | 36.6 | 69.0 | NADH >> NAPDH | 5.19 | 31.5 | 1.46 | 36.5 | 3.3 | 6.0 |
| C. tropicalis (61) | 36.5 | 58.0 | NADPH | 4.15 | 30 to 37 | —a | 9 to 14 | — | 6.0 |
| C. tenuis (44) | 43 | 48 | NADPH > NADH | 4.70 | 72 | 0.30 | 4.8 | 25.4 | 6.0 |
| S. cerevisiae (34) | 33 | 33 | NADPH | 6.60 | 27.9 | 0.12 | 0.013 | — | 5.0 |
| P. stipitis (58) | 34 | 65 | NADPH > NADH | 5.76 | 42 | 0.60 | 9 | 21 | 6.0 |
| P. tannophilus (19) | 38 | 38 | NADPH | 4.87 | 162 | 0.062 | 59 | — | 7.0 |
a
—, not reported.