FIG. 3.

C. salexigens requires Cl⁻ ions for growth. The cells were grown as described in the legend to Fig. 2. Growth rates were determined after a second subculture into M63-5× Mg-Fe-10 mM glucose containing the indicated concentrations of Cl⁻ and NO₃⁻, added as Na⁺ salts. The final density of all cultures was ∼2 × 10⁹ cells/ml (A₆₀₀ = 1.2 to 1.5), except for those containing ≤0.1 M NaCl (in which case there was no growth).