TABLE 1.
Description of strains and plasmids used in this study
| Strains and plasmids | Description | Reference or source |
|---|---|---|
| Strains | ||
| E. coli KO11 | Δfrd, CmR, carrying the Zymomonas mobilis pdc adhB cassette | 38 |
| E. coli TOP10F′ | lacIqlacZΔM15 | Invitrogen |
| K. oxytoca M5A1 | Wild type | 37 |
| Plasmids | ||
| pCR2.1-TOPO | 3.9 Kbp, KmR AmpR, pUC origin, TA cloning vector | Invitrogen |
| pNEB193 | AmpR cloning vector similar to pUC19 | New England Biolabs |
| pLOI3701 | pUC18 derivative with ∼7-kbp DNA fragment, xynT′ xynB | This study |
| pLOI3702 | pUC18 derivative with ∼6.3-kbp DNA fragment, xynT′ xynB | This study |
| pLOI3703 | pUC18 derivative with ∼5.6-kbp DNA fragment, xynT′ xynB | This study |
| pLOI3704 | pCR2.1-TOPO derivative with ∼6.0-kbp DNA fragment, xynT′ xynB | This study |
| pLOI3705 | pCR2.1-TOPO derivative with ∼6.0-kbp DNA fragment, xynTB operon | This study |
| pLOI3706 | derivative of pLOI3705 with all Klebsiella DNA removed between the two vector EcoRI sites | This study |
| pLOI3707 | pNEB193 derivative carrying the 3.6-kbp AseI-PstI fragment with the complete xynTB coding regions (lacking regulatory sites); transcribed opposite to the lac promoter | This study |
| pLOI3708 | pNEB193 derivative containing the 3.6-kbp AseI-PstI fragment with the complete xynTB coding regions (lacking regulatory sites); transcribed in the same direction as the lac promoter | This study |
| pLOI3709 | Derivative of pLOI3708, ΔxynT, xynB (918-bp ClaI internal deletion of xynT) | This study |