TABLE 2.

β-Xylosidase activities in permeabilized cell preparations of K. oxytoca M5A1 and recombinant E. coli^a

Strains and properties	Growth media	β-Xylosidase activity (nmol min−1 mg−1)
K. oxytoca M5A1	LB + 5% glucose	0.5
	LB + 2.5% glucose + 2.5% xylose	0.5
	LB + 5% xylose	28
E. coli KO11(pLOI3705)	LB + 5% glucose	7.5
	LB + 2.5% glucose + 2.5% xylose	43
	LB + 5% xylose	140 (5.0)
E. coli TOP10F′ (pLOI3708)	LB, no sugar, without IPTG	520
	LB, no sugar, with IPTG	3,100 (105)
E. coli KO11(pLOI3708)	LB + 5% xylose	1,020
E. coli KO11(pLOI3709)	LB + 5% xylose	1,100
E. coli KO11 (pNEB193)	LB + 5% xylose	0.4
E. coli TOP10F′	LB, no sugar	<0.4

^aValues represent an average of two or more experiments which differed by less than 25%. Arabinosidase activities are shown in parentheses.