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Time course of recombinant XynA expression in 250 ml of optimized medium BMM(M5) inoculated with P. pastoris clone 7. Cell counts (▪) and xylanase activity (□) were monitored throughout the experiment. Xylanase activity was measured by the DNS procedure as described in Materials and Methods. The values correspond to the averages of five measurements, and the error was below 10%.
