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. 2003 Oct;69(10):6064–6072. doi: 10.1128/AEM.69.10.6064-6072.2003

TABLE 2.

Effect of medium (M1 to M8) composition on the production of recombinant XynA by P. pastoris after 48 h of induction in 5-ml cultures

Medium Composition
Xylanase activity (U/ml)a
Methanol (%, vol/vol) YNB (%, wt/vol) Initial cell density (108 cells/ml)
M1 0.5 0.9 0.5 157
M2 1.0 0.9 0.5 152
M3 0.5 1.8 0.5 156
M4 1.0 1.8 0.5 168
M5 0.5 0.9 5.0 305
M6 1.0 0.9 5.0 360
M7 0.5 1.8 5.0 340
M8 1.0 1.8 5.0 264
a

Xylanase activity is the average of three experiments (DNS procedure).