FIG. 1.
Expression plasmids for lysis gene E and the staphylococcal nuclease (SNUC). The vectors are presented as plasmid combinations utilized for the coexpression of gene E and SNUC in E. coli NM522. The expression of the killing genes was triggered by either the addition of an inducer (+IPTG) or a thermal upshift from 28°C to 42°C (28°C ⇒ 42°C). Amp, ampicillin resistance gene; Kan, kanamycin resistance gene; Tet, tetracycline resistance gene; colE1 and p15A, origins of replication; A1-O4/O3, synthetic, chemically inducible promoter; pRM/pR, rightward “maintenance” and rightward promoters of bacteriophage lambda; cI857, gene encoding the thermosensitive repressor for the lambda pR promoter; EcoRI, restrictions sites used for the construction of pHSSNUC.