TABLE 3.
Activities of lectins of various specificities for C. diphtheriae strains grown under standard and iron-limited conditionsa
| Lectin specificity | Minimum lectin conc (μg ml−1) for detectable fluorescence of strain:
|
Effect of iron depletion on lectin binding | |||
|---|---|---|---|---|---|
| CDC-E8392 (non-sucrose fermenting)
|
241 (sucrose fermenting)
|
||||
| TSB | TSB − Fe | TSB | TSB − Fe | ||
| d-GlcNac-binding lectins | |||||
| Triticum vulgaris (wheat germ agglutinin) | 62.5 | 250.0 | 15.6 | 250.0 | Decrease |
| Bandeiraea simplicifolia (BS-II) | 31.2 | 31.2 | 3.9 | 3.9 | No effect |
| Solanum tuberosum (STA) | 250.0 | 250.0 | 125.0 | 125.0 | No effect |
| d-GalNAc-binding lectins | |||||
| Glycine max (SBA) | 31.2 | 31.2 | 125.0 | 125.0 | No effect |
| Wisteria floribunda (WFH) | 250.0 | 62.5 | 250.0 | 31.5 | Increase |
| Artocarpus integrifolia (JACA) | 31.2 | 31.2 | 125.0 | 125.0 | No effect |
| d-Gal-binding lectins | |||||
| Euonymus europaeus (EEL) | >500b | 125.0 | >500 | 125.0 | Increase |
| Arachis hypogaea (PNA) | >500 | 62.5 | >500 | 15.6 | Increase |
| Ricinus communis I (RCA-I) | 62.5 | 15.6 | 250.0 | 31.2 | Increase |
| d-Man-binding lectins | |||||
| Canavalia ensiformis (ConA) | 62.5 | 7.8 | 125.0 | 3.9 | Increase |
| Lens culinaris (LCL) | 125.0 | 31.2 | 250.0 | 15.6 | Increase |
| Pisum sativum (PSA) | 125.0 | 31.2 | 250.0 | 15.6 | Increase |
| Sialic acid-binding lectin, Sambucus nigra (SNA) | 62.5 | 250.0 | 15.6 | 250.0 | Decrease |
a
Results are average from three experiments. TSB, bacterial growth in standard TSB medium; TSB − Fe, TSB medium with 0.5 mM dypiridyl.
b
No fluorescence was seen at the maximum concentration used.