Figure 5.
Comparison of Kupffer cell function in fa/fa and ?/fa rats. (A) Total liver RNA was isolated from two distinct rats/group either before (pre) or 1 and 24 h after LPS (0.5 μg/g), separated by electrophoresis on denaturing polyacrylamide gels (20 μg RNA/lane), transferred to nylon membranes, and cohybridized with [32P]-labeled cDNAs for KCR and a constitutively expressed monocyte transcription factor (Pu-1). (B and C) Rats were injected intraportally with fluorescent beads, and intravital microscopy was used to evaluate (B) the distribution of beads in acinar zones 1 (periportal), 2 (midacinus), and 3 (pericentral) 30 minutes after injection and (C) the cumulative hepatic uptake of the beads over time. Results shown are representative of replicate experiments.