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(A) Schematic diagram of the baculovirus expression constructs for human FSHRHB and the covalently linked human FSHα and FSHβ subunits.
(B) Western blot of the culture medium of insect cells that were infected with the recombinant virus for FSHRHB and single-chain FSH. Culture samples were concentrated 20-fold and run on a 4-15% SDS gel in the presence of 100 mM dithiothreitol (DTT). The blot was developed using the M2 anti-FLAG antibody as the primary antibody.
