Fig. 2.
(A) Gel filtration column chromatography of the FSH-FSHRHB complex. Samples of the indicated peak fraction were assayed on (B) 8–25% SDS gel and (C) 8–25% native gel. Samples of purified FSH-FSHRHB (5 μg) and free hormone ligands were also analyzed by 8–25% native gel electrophoresis for possible additional interactions upon mixture: (D) for 5 μg of free CG, and (E) for 7.5 μg of free FSH.