Skip to main content
. Author manuscript; available in PMC: 2007 Oct 10.
Published in final edited form as: Protein Eng Des Sel. 2006 Jan 19;19(3):113–119. doi: 10.1093/protein/gzj010

Table I.

Directed evolution of Synechococcus PCC6301 rbcL

Round [CO2]a 1° Selectionb 2° Screenb 3° Screenb Hypermorphs
1 9–54 mM 2 × 105 70 26 1
2 9–54 mM 7 × 104 44 12 6
3 9–27 mM 3 × 105 70 12 5
a

Each round of selection was carried out under different concentrations of carbon dioxide. The actual intracellular CO2 concentration is not known, but assumed to reflect the weight of dry ice sublimated in 4 l ziploc bags. All of these values are >100× atmospheric partial pressures.

b

Estimated number of clones evaluated at each step.