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. Author manuscript; available in PMC: 2007 Oct 10.
Published in final edited form as: Mol Biol Evol. 2006 Jan 19;23(4):764–772. doi: 10.1093/molbev/msj098

Fig. 4.

Fig. 4

Expression levels of HIV PRs. E. coli transformed with WT, D25N or P9S/I50L HIV PR expression vectors were propagated to mid logarithmic phase in LB-ampicillin media. Gene expression was induced with 1 mM IPTG for 30 minutes. A) Whole cells were harvested by centrifugation, resuspended in 1 ml 1× SDS-PAGE running buffer, and analyzed by western blot analysis using an anti-HIV PR antibody. An IRD800 labeled secondary antibody and a LICOR Odyssey scanner were used to quantify the protein in each band. B) Cells were lysed, and the soluble fraction was removed and analyzed by western blot analysis.