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. Author manuscript; available in PMC: 2007 Oct 10.
Published in final edited form as: Mol Biol Evol. 2006 Jan 19;23(4):764–772. doi: 10.1093/molbev/msj098

Fig. 5.

Fig. 5

Activity of purified proteases against fluorogenic peptide substrates. The wild-type (WT) PR was purified, and equimolar concentrations (191 nM) were separately reacted with 1.45–13.85 μM native HIV PR substrate I. The initial velocities of product formation were recorded for up to 20 minutes in a fluorescence spectrophotometer. These rates were then converted to velocity in units of concentration per minute and plotted against substrate concentration.