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. Author manuscript; available in PMC: 2007 Oct 10.
Published in final edited form as: Mol Biol Evol. 2006 Jan 19;23(4):764–772. doi: 10.1093/molbev/msj098

Table 1.

In Vivo Coupled Assays of HIV PR Activity

Wild-Type BGAL p6/BGAL TNF-α/BGAL
Wild-type HIV PR 1.966 ± 0.03a 0.834 ± 0.01 1.053 ± 0.06
D25N HIV PRb 2.063 ± 0.09 1.060 ± 0.04 1.384 ± 0.03
P9S/I50L HIV PR 1.884 ± 0.04 1.000 ± 0.08 0.687 ± 0.02
a

The OD405 was monitored for 5 h in a microplate spectrophotometer, and the activities (in units of change in mAbs405 per hour per cell) are reported.

b

All values should be compared to those of the catalytically inactive D25N (negative control), which should have little effect upon BGAL activity.