Table 2.
IL-1β produced in HUVECs and THP-1 monocytes measured by ELISA
| Cell type | Treatment | IL-1β (pg/ml) |
|---|---|---|
| Mean±s.e.m. (n=3) | ||
| HUVECs | Untreated | 0 |
| LPS | 124±65 | |
| IFNγ | 0 (NS) | |
| TNFα | 86±29 | |
| IFNγ/TNFα | 158±92 | |
| IFNγ/LPS | 410±113 | |
| TNFα/LPS | 708±330 (NS) | |
| IL-1β | 611±92 | |
| IL-1β/IFNγ | 821±73 | |
| IL-1β/TNFα | 1880±477 | |
| IL-1β/LPS | 1570±565 | |
| THP-1 monocytes | Untreated | 0 |
| LPS | 8750±751 |
Levels of IL-1β produced in HUVECs and THP-1 monocytes following treatment with various combinations of stimuli. IL-1β levels from whole cell lysates were determined by the R&D QuantiGlo ELISA kit. Values are the mean±s.e.m. for n=3 separate determinations; P<0.05; unpaired Student's t-test, except where shown as NS.
Abbreviations: ELISA, enyme-linked immunosorbent assay; HUVEC, human umbilical vein endothelial cell; IFNγ, interferon γ; IL-1, interleukin-1; LPS, lipopolysaccharide; NS, nonsignificant; TNFα, tumour necrosis factor-α.