Figure 3.

Dissociation of [³⁵S]GTPγS binding carried out in the presence of different agonists. Dissociation of [³⁵S]GTPγS binding was carried out in the presence of a range of agonists as described in the Materials and methods section. Each agonist was present at a maximally effective concentration throughout the experiment that is, during [³⁵S]GTPγS association and dissociation. Agonists tested were 1 mM dopamine, 1 mM quinpirole, 10 μM bromocriptine, 1 mM p-tyramine, 1 mM (+)-3-PPP and 10 μM NPA. Data are expressed as a percentage of [³⁵S]GTPγS binding at time zero after the subtraction of basal levels of [³⁵S]GTPγS dissociation. Dissociation data were fitted best by a model of a single exponential decay. Data shown are representative curves for experiments replicated at least three times.