Table 3.
Effect of increasing CaCl2 concentration on the affinity (pKL), nH and Bmax of [3H]clobenpropit at histamine H3-receptors in guinea-pig cerebral cortex
| Buffer | pKL | Bmax (fmol mg−1) | nH |
|---|---|---|---|
| B(0,0,0) | 10.34±0.10 | 3.19±0.25 | 1.23±0.11 |
| B(0.03, 0,0) | 10.16±0.22 | 3.54±0.05 | 1.11±0.05 |
| B(0.07,0,0) | 10.05±0.06 | 4.17±0.38 | 1.08±0.09 |
| B(0.1,0,0) | 9.84±0.15 | 4.14±0.23 | 1.04±0.14 |
| B(0.2,0,0) | 9.70±0.05 | 3.53±0.32 | 1.06±0.12 |
| B(0.3,0,0) | 9.60±0.02 | 4.10±0.23 | 1.06±0.02 |
Data are the mean±s.e.m. of three separate experiments. The concentrations of CaCl2 used in the buffers were (M): 0, 0.03, 0.07, 0.1, 0.2, 0.3. The s.e.m. of the nH parameter from individual data sets was between 0.36 and 0.09.