Table 1.
Resting dimension of isolated ductus arteriosus from WT and gene-deleted term fetal mice
| Genotype | Internal diameter |
Vessel lengtha |
Wall thickness | |
|---|---|---|---|---|
| Short side | Long side | |||
| WT (n=61) | 139±1 | 532±8 | 545±8 | 19±0.3 |
| eNOS−/− (n=27) | 132±1* | 545±10 | 558±9 | 20±0.4 |
| iNOS−/− (n=14) | 152±2** | 553±10 | 569±10 | 19±0.7 |
| COX1−/− (n=7) | 130±1 | 501±34 | 510±34 | 21±0.7 |
| COX2−/− (n=7) | 131±2 | 460±14 | 473±13 | 18±0.4 |
| HO-2−/− (n=10) | 133±2 | 511±29 | 523±28 | 19±0.8 |
Abbreviations: ANOVA, analysis of variance; COX, cyclooxyygenase; eNOS, endothelial nitric oxide synthase; HO, heme oxygenase; iNOS, inducible nitric oxide synthase; WT, wild type.
Values (μm) are mean±s.e.m.
a
Vessel length of the ductus arteriosus is uneven because of its insertion into aorta at an angle. Short side was taken for normalization of internal circumference and calculation of tension output (see in vitro studies). *P<0.01, **P<0.001 vs WT (ANOVA with Bonferroni post hoc test). Note that the internal diameter of the ductus was smaller in eNOS−/− than WT, whereas the reverse occurred with iNOS−/−.
*
P<0.01
**
P<0.001