Table 2.
Isolated ductus arteriosus from term fetal mice: wall tension before bradykinin-induced relaxation
| Condition |
Genotype |
||
|---|---|---|---|
| WT | eNOS−/− | iNOS−/− | |
| Indomethacin | 0.88±0.11 (10) | 1.05±0.08 (6) | 0.71±0.11 (6) |
| Indomethacin+L-NAME | 0.99±0.06 (5) | — | — |
| Indomethacin+ZnPP | 0.79±0.09 (6) | 0.90±0.12 (4) | — |
| Indomethacin+L-NAME+ZnPP | 1.20±0.09 (4) | — | — |
| Indomethacin+L-NAME+ZnPP+K+ 20 mM | 0.76±0.09 (4) | — | — |
| Indomethacin+L-NAME+ZnPP+K+ 55 mM | 1.0±0.18 (4) | — | — |
| Indomethacin+L-NAME+ZnPP +TRAM-34+apamin | 1.21±0.18 (4) | — | — |
| Indomethacin+TRAM-34+apamin | — | 0.52±0.05 (7)* | — |
| Indomethacin+ZnPP+TRAM-34+apamin | — | 0.61±0.03 (3)** | — |
Abbreviations: ANOVA, analysis of variance; eNOS, endothelial nitric oxide synthase; iNOS, inducible nitric oxide synthase; L-NAME, NG-nitro-L-arginine methyl ester, TRAM-34, 1-[(2-chlorophenyl)diphenylmethyl]-1H-pyrazole; WT, wild-type; ZnPP, zinc protoporphyrin.
Values (mN mm−1) are mean±s.e.m. and apply to the steady tension of the preparation. Number of experiments in parentheses. Ductus preparations from WT and NOS-deleted animals were treated with indomethacin alone or in combination with excess potassium and the required inhibitors in various combinations (for details, see text).
*P<0.01, **P<0.05 vs indomethacin alone in the same genotype (ANOVA with Bonferroni post hoc test). Note that differences in the indomethacin response among genotypes are not significant.
P<0.01
P<0.05