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. 1997 Mar 18;94(6):2609–2614. doi: 10.1073/pnas.94.6.2609

Figure 2.

Figure 2

Nfil3/E4bp4 expression is regulated by IL-3. (A) Northern blot analysis of total RNA (20 μg per lane) prepared from Baf-3 (Upper) or FL5.12 (Lower) pro-B lymphocytes were grown in IL-3 (lane 1) and then deprived of the ligand for the indicated intervals (lanes 2–5). Cells starved of ligand for 8 hr (lane 7) were restimulated with 10 ng/ml of murine IL-3 and incubated for the indicated times (lanes 8–11). Blots were hybridized with an Nfil3/E4bp4 probe and stripped and rehybridized with the GAPDH control probe. (B) Induction of Nfil3/E4bp4 by IL-3 requires protein synthesis. Asynchronously growing FL5.12 cells were deprived of IL-3 for 16 hr (lane 1) and stimulated with either IL-3 alone for the indicated times (lanes 2–6), or with cycloheximide (CHX, 20 μg/ml) alone (lanes 7–11), or were pretreated with CHX for 15 min and incubated in both IL-3 and CHX for the indicated times (lanes 12–16). Northern blots were sequentially hybridized with probes for Nfil3/E4bp4, c-fos, and GAPDH.