TABLE 4.
Rescue of D14-3-3ε lethality with leo transgenes
| Genotype | Temperature | % viable | % rescue | n |
|---|---|---|---|---|
| D14-3-3εl(3)j2B10/D14-3-3εl(3)j2B10 | 18° | 75 | — | 645 |
| D14-3-3εl(3)j2B10/D14-3-3εl(3)j2B10 | 23° | 77 | 8 | 526 |
| D14-3-3εl(3)j2B10/D14-3-3εl(3)j2B10 | HS | 78 | 12 | 522 |
| D14-3-3εl(3)j2B10/D14-3-3εl(3)j2B10, hsleoIL | 18° | 76 | — | 462 |
| D14-3-3εl(3)j2B10/D14-3-3εl(3)j2B10, hsleoIL | 23° | 84 | 33 | 455 |
| D14-3-3εl(3)j2B10/D14-3-3εl(3)j2B10, hsleoIL | HS | 88 | 50 | 524 |
| D14-3-3εl(3)j2B10/D14-3-3εl(3)j2B10, hsleoIIL | 18° | 78 | — | 442 |
| D14-3-3εl(3)j2B10/D14-3-3εl(3)j2B10, hsleoIIL | 23° | 95 | 77a | 492 |
| D14-3-3εl(3)j2B10/D14-3-3εl(3)j2B10, hsleoIIL | HS | 100 | 100a | 425 |
| D14-3-3εex4/D14-3-3εex4 | 18° | 42 | — | 382 |
| D14-3-3εex4/D14-3-3εex4 | 23° | 45 | 5.5 | 411 |
| D14-3-3εex4/D14-3-3εex4 | HS | 45 | 5.5 | 409 |
| D14-3-3εex4/D14-3-3εex4, hsleoIL | 18° | 49 | — | 481 |
| D14-3-3εex4/D14-3-3εex4, hsleoIL | 23° | 56 | 14 | 440 |
| D14-3-3εex4/D14-3-3εex4, hsleoIL | HS | 87 | 74.5a | 493 |
| D14-3-3εex4/D14-3-3εex4, hsleoIH | 18° | 51 | — | 415 |
| D14-3-3εex4/D14-3-3εex4, hsleoIH | 23° | 60 | 18.5 | 433 |
| D14-3-3εex4/D14-3-3εex4, hsleoIH | HS | 85 | 69a | 495 |
| D14-3-3εex4/D14-3-3εex4, hsleoIIL | 18° | 49 | — | 496 |
| D14-3-3εex4/D14-3-3εex4, hsleoIIL | 23° | 69 | 39a | 534 |
| D14-3-3εex4/D14-3-3εex4, hsleoIIL | HS | 100 | 100a | 555 |
| D14-3-3εex4/D14-3-3εex4, hsleoIIH | 18° | 56 | — | 512 |
| D14-3-3εex4/D14-3-3εex4, hsleoIIH | 23° | 91 | 76.5a | 534 |
| D14-3-3εex4/D14-3-3εex4, hsleoIIH | HS | 100 | 100a | 485 |
| D14-3-3εex4/D14-3-3εex4,UASleoI | 25° | 37 | — | 230 |
| elavGal4/+ ; D14-3-3εex4/D14-3-3εex4,UASleoI | 25° | 63 | 41a | 331 |
| D14-3-3εex4/D14-3-3εex4,UASleoII | 25° | 34 | — | 248 |
| elavGal4/+; D14-3-3εex4/D14-3-3εex4,UASleoII | 25° | 100 | 100a | 380 |
leo transgenes rescue the lethality of D14-3-3ε mutant homozygotes. The temperature conditions employed for the crosses are shown under “Temperature.” Under HS conditions, flies were raised under constant 23°, except for three daily 30-min, 32° heat shocks. Viability was calculated as the fraction of adults of each genotype recovered from crosses of balanced individuals over that expected if the mutant homozygotes were fully viable. “% rescue” was calculated as the increase in mutant homozygotes carrying transgenes over the “baseline” number (—). The superscripts H and L for leoI and leoII transgenes denote high- and low-expressing transgenes, respectively. n denotes the total number of flies scored per cross that yielded the mutant homozygotes carrying D14-3-3ε or leo transgenes.
Significant rescue.