TABLE 5.
Wing cross-vein deficits of D14-3-3ε mutants and transgenic rescue
| Genotype | % anterior malformed | % posterior malformed | % rescue |
|---|---|---|---|
| A. | |||
| D14-3-3εex5/D14-3-3εex5 | 0 | 0 | — |
| D14-3-3εex5/D14-3-3εl(3)j2B10 | 0 | 0 | — |
| D14-3-3εex5/D14-3-3εex4 | 0 | 0 | — |
| D14-3-3εl(3)j2B10/D14-3-3εl(3)j2B10 | 25.0 | 75.0 | — |
| D14-3-3εl(3)j2B10/D14-3-3εex4 | 26.7 | 82.4 | — |
| D14-3-3εex4/D14-3-3εex4 | 42.9 | 80.9 | — |
| B. | |||
| D14-3-3εex4/D14-3-3εex4, hsD14-3-3εL (18°) | 40.6 | 83 | — |
| D14-3-3εex4/D14-3-3εex4, hsD14-3-3εL | 9.3a | 14a | 82.3 |
| D14-3-3εex4/D14-3-3εex4, hsD14-3-3εH (18°) | 41.8 | 79.5 | — |
| D14-3-3εex4/D14-3-3εex4, hsD14-3-3εH | 0a | 3.6a | 95.6 |
| C. | |||
| D14-3-3εex4/D14-3-3εex4, hsleoIL (18°) | 43.2 | 82.2 | — |
| D14-3-3εex4/D14-3-3εex4, hsleoIL | 29a | 72 | 11 |
| D14-3-3εex4/D14-3-3εex4, hsleoIH (18°) | 41.3 | 80.9 | — |
| D14-3-3εex4/D14-3-3εex4, hsleoIH | 0a | 60 | 25.8 |
| D14-3-3εex4/D14-3-3εex4, hsleoIIL (18°) | 43.4 | 82.6 | — |
| D14-3-3εex4/D14-3-3εex4, hsleoIIL | 40 | 78.4 | 3.1 |
| D14-3-3εex4/D14-3-3εex4, hsleoIIH (18°) | 41.5 | 81.8 | — |
| D14-3-3εex4/D14-3-3εex4, hsleoIIH | 33 | 76.8 | 5.1 |
The wing cross-vein deficits are rescued by conditional hsD14-3-3ε expression, but not by leo transgenes. (A) Percentage of D14-3-3ε controls and mutant homozygotes that exhibited posterior and anterior cross-vein deficits. Posterior and anterior cross-vein deficits were counted on the same wings and one wing was scored per individual (n > 100). (B) The percentage of D14-3-3εex4/D14-3-3εex4, hsD14-3-3ε exhibiting wing cross-vein deficits raised under conditions of transgene silence (18°) or induction. The “% rescue” indicates the percentage decrease in individuals with deficient wings and is shown only for posterior cross veins. (C). The percentage of D14-3-3εex4/D14-3-3εex4 individuals expressing leoI and leoII transgenes exhibiting wing cross-vein deficits raised under conditions of transgene silence (18°) or induction. The “% rescue” indicates the percentage decrease in individuals with deficient wings and is shown only for posterior cross veins.
Large changes in the percentage of deficient wings (rescue).