TABLE 1.
Free-running locomotion, in DD or LL, at different temperatures
| Photic condition | 18°
|
25°
|
29°
|
|||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Genotype | N | N rhy (%) | Mean hr ± SEM | N | N rhy (%) | Mean hr ± SEM | N | N rhy (%) | Mean hr ± SEM | |
| DD | s-tim cry01 or 02 | 82 | 51 (62) | 23.7 ± 0.1 | 51 | 50 (98) | 23.7 ± 0.1 (82% of rhy) or 22.9 ± 0.1 (18% of rhy) | 55 | 52 (95) | 23.4 ± 0.1 |
| DD | s-tim cry+ | 16 | 15 (94) | 23.9 ± 0.1 | 14 | 14 (100) | 23.9 ± 0.0 | 29 | 29 (100) | 23.4 ± 0.1 |
| LL | s-tim cry01 or 02 | 89 | 55 (62) | 24.2 ± 0.1 | 74 | 70 (95) | 21.4 ± 0.0 and 25.4 ± 0.1 | 85 | 48 (57) | 20.1 ± 0.1 and 23.8 ± 0.2 |
| LL | ls-tim cry01 or 02 | ND | 67 | 57 (85) | 21.7 ± 0.0 and 25.4 ± 0.1 | ND | ||||
| LL | Df/cry01, 02, or 03 | ND | 63 | 58 (92) | 23.6 ± 0.1 (38% of rhy) 21.9 ± 0.2 and 25.0 ± 0.1 (62% of rhy) | ND | ||||
Flies were entrained under 12:12 hr light-dark cycling conditions for 5–6 days then released into constant conditions for 7 days of locomotor monitoring. s-tim and ls-tim, naturally occurring tim iso-alleles (Rosato et al. 1997; Peschel et al. 2006); Df, deletion of the cry locus and of neighboring third-chromosomal genes; N, numbers of tested flies or number rhythmic (rhy); Mean, average period determined by periodogram and verified by the straight-edge/slope procedure (see materials and methods); SEM, standard error of the mean; ND, not done (meaning that the LL monitorings of flies with genotypes in the bottom two rows were tested only at 25°). No differential effects on these characters were discernible with regard to cry0 derived from the separately numbered such strains (second column from the left).