Figure 4.—

The presence of Taf3p at HSP12 and SNZ1 promoters increases in not1-2 and this is suppressed by the deletion of SPT3. The indicated strains were grown as in Figure 3 to an OD₆₀₀ of 0.8 at 37.5°, and the cells were crosslinked prior to total extract preparation and immunoprecpitation with the indicated antibodies. The amount of indicated promoter DNAs in the immunoprecipitates was evaluated by real-time PCR, expressed relative to input DNA, and then normalized to the amount of an intergenic unexpressed DNA. (A) The antibodies were against H3-K4-Me₃ and the results presented are the average of two experiments performed with independent cultures. (B) Antibodies were against Taf3p and the experiment was repeated three times with independent cultures. (C) Antibodies against Taf9p and Taf11p were used and the relative amount of DNA immunoprecipitated with Taf9p to that immunoprecipiated with Taf11p was calculated (Taf9p/Taf11p). The experiment was repeated two times with independent cultures. (D) The indicated strains expressed MYC-tagged Spt7p and antibodies against MYC were used. The experiment was repeated three times with independent cultures.