Figure 6.—
Localization of Cdc14 during sporulation. (A) Wild type (CDC15, left) or PCLB2-CDC15 mutant (cdc15, right) were transferred to sporulation medium. At different times after the induction of sporulation, cells were collected and stained with anti-Cdc14 and anti-tubulin antibodies and DAPI. Anti-goat Alexa Fluor 488 and anti-mouse Alexa Fluor 633 were used as secondary antibodies. Cdc14 (green), tubulin (red), and DAPI (blue) images are shown, as well as the merged image. Representative images of the different meiotic phases are shown for each strain. (B) Localization of Cdc14 during the different stages of the sporulation in wild-type (left) or PCLB2-CDC15 (right) cells. Localization of Cdc14 in each phase of the sporulation process was classified as sequestered in the nucleolus (blue), fully (maroon) or partially (yellow) released from the nucleolus, free in the nucleus (light blue), or cells showing no staining (black). For wild-type cells, the different localization is presented as a percentage of metaphase I (MI; n = 109), anaphase I (AI; n = 92), metaphase II (MII; n = 149), anaphase II (AII; n = 155), or spores (n = 113) with visible Cdc14. For PCLB2-CDC15 cells, a similar percentage for metaphase I (MI; n = 112), anaphase I (AI; n = 74), metaphase II (MII; n = 128), anaphase II (AII; n = 132), or postmeiotic cells (n = 64) is shown.