TABLE 3.

Two-hybrid interactions between Shs1 and other septins

	DBD fusion
AD fusion	Cdc3	Cdc10	Cdc11	Cdc12	Shs1	Control
Shs1	1.15	0.52	1.96	62.07	0.03	0.07
Control	0.03	0.05	0.07	0.03	0.03	0.06

AD-Shs1 means pM-25. DBD fusion plasmids are LexA DBD-Cdc3 (pM-15), LexA DBD-Cdc10 (pM-16), LexA DBD-Cdc11 (pM-17), LexA DBD-Cdc12 (pM-19), and LexA DBD-Shs1 (pM-20). Each pair of plasmids were introduced into J21, and β-galactosidase activity (units) was determined as described in materials and methods.