Figure 1.—

Subito localization to the central spindle is microtubule dependent. (A) Wild-type stage 14 oocyte treated in buffer without colchicine. (B and C) Stage 14 oocytes treated with colchicine for 1 hr. DNA is in blue, Subito in red, and tubulin in green. The Subito and Tubulin channels are shown below the merged images. Note that the brightest tubulin fluorescence is present in the central spindle of the control spindle, but this region shows the least intense fluorescence when colchicine treated. Subito is either absent or uniformly localized following colchicine treatment. The high background in B is due to optimizing on the low Subito signal. Bar, 10 μm.