Figure 4.—

Localization of Subito in stage 14 oocytes of motor domain mutants. In all the experiments discussed below, the transgenes were expressed using the P{GAL4∷VP16-nos.UTR}MVD1 driver. DNA is in blue, Subito in red, and tubulin in green. The Subito channel is shown below each color image. (A) Subito detected using an anti-Subito antibody in a wild-type oocyte. (B) Subito detected using an anti-Subito antibody in a sub^Dub/sub¹ oocyte. (C) Subito detected using an antibody to the HA tag in an oocyte expressing wild-type sub fused to an HA epitope tag (P{UASP:sub^+HA}) or (D) a similar HA-fusion protein with a mutation in the ATP binding domain (P{UASP:sub^ATP}) in a wild-type background or (E) sub^ATP in a sub^null mutant background. (F) Subito detected using an anti-HA antibody in an oocyte expressing a HA-fusion protein lacking 60 amino acids present in the motor domain of all kinesin-6 family members (P{UASP:sub^L6}) in a wild-type background. (G) Subito detected using an anti-Subito antibody in sub⁺; P{UASP: sub^+HA} or (H) sub⁺; P{UASP:sub^ATP} oocytes. Bar, 10 μm.