TABLE 1.
Statistically significant overrepresented GO terms per ovarian fertility factor class
| Class IA: follicle assembly/meiosis |
| Biological process |
| Meiotic cell cycle (P = 0.003) |
| M phase of meiotic cell cycle (P = 0.003) |
| Meiosis (P = 0.003) |
| Synapsis (P = 0.003) |
| Molecular function |
| Transcriptional regulator activity (P = 0.008) |
| Nucleic acid binding* (P = 0.0009) |
| DNA binding* (P = 0.0004) |
| Cellular component |
| Condensed nuclear chromosome* (P = 0.0003) |
| Synaptonemal complex* (P = 0.0001) |
| Transcription elongation factor complex* (P = 7 × 10−6) |
| Class IB: oocyte-specific, early and late maturation |
| Biological process |
| Embryonic pattern specification (P = 0.009) |
| Reproduction* (P = 0.0001) |
| Fertilization* (P = 0.0003) |
| Sperm egg recognition (P = 0.002) |
| Binding of sperm to zona pellucida (P = 0.002) |
| Molecular function |
| Nucleic acid binding (P = 0.007) |
| RNA binding (P = 0.003) |
| mRNA binding (P = 0.007) |
| Molecular function unknown (P = 0.003) |
| Cellular component |
| Pronucleus (P = 0.001) |
| Class IC: oocyte-specific, early maturation only |
| Biological process |
| Reproduction* (P = 6 × 10−5) |
| Fertilization* (P = 3 × 10−6) |
| Sperm-egg recognition* (P = 8 × 10−5) |
| Binding of sperm to zona pellucida* (P = 8 × 10−5) |
| Cellular component |
| ECM (P = 0.001) |
| Class ID: follicle growth, somatic |
| Biological process |
| Cell adhesion (P = 0.009) |
| Development* (P = 0.0006) |
| Sex differentiation (P = 0.003) |
| Molecular function |
| ECM structural constituent (P = 0.004) |
| Metalloendopeptidase activity (P = 0.009) |
| Transmembrane receptor protein kinase activity (P = 0.004) |
| Cellular component |
| ECM* (P = 0.0005) |
| Class IE: follicle growth, somatic, with high adrenal expression |
| Biological process |
| Steroid metabolism (P = 0.001) |
| Steroid biosynthesis* (P = 0.0003) |
| C21-steroid hormone biosynthesis* (P = 1 × 10−5) |
| Class II: ovary |
| Biological process |
| Reproductive physiological process (P = 0.002) |
| Cellular lipid metabolism* (P = 0.0002) |
| Steroid metabolism* (P = 3 × 10−5) |
| C21-steroid biosynthesis* (P = 8 × 10−9) |
| Molecular function |
| Lipid binding (P = 0.004) |
| Steroid binding (P = 0.002) |
| Steroid hydroxylase activity* (P = 6 × 10−5) |
| Endopeptidase inhibitor activity* (P = 0.0009) |
| Class III: egg |
| Biological process |
| Development (P = 0.007) |
| Organ morphogenesis (P = 0.008) |
| Reproduction (P = 0.002) |
| BMP signaling pathway (P = 0.009) |
| Retinoic acid metabolism* (P = 0.0006) |
| Molecular function |
| Polysaccharide binding (P = 0.009) |
| Hyaluronic acid binding (P = 0.004) |
Parent–child relationships are shown by indented lines. All terms for which P < 0.01 are shown; *P < 0.001. GO analysis was performed using the WebGestalt gene set analysis tool kit; Affymetrix identifiers were used for gene set retrieval. Gene set analysis and statistical comparisons were done against the 430 2.0 reference set, with P-values calculated by the hypergeometric test. Criteria for enriched GO categories were a minimum of two genes at a 0.01 significance level.