Figure 1.—

Derivation and phenotype of strains. A schematic of selection used to derive JWY101 and JWY102 after Cakar et al. (2005) is shown. JWY100 is an isogenic, clonal derivative of CEN.PK 113-14A. Cakar et al. (2005) performed the selection for stress-resistant populations as follows. The ancestral population was EMS mutagenized and then treated with H₂O₂ for 1 hr. The surviving population recovered overnight in minimal medium (YMM). This population was EMS mutagenized and split into two lines. One line was exposed to heat stress followed by overnight recovery in YMM and EMS mutagenesis and freeze/thaw stress (H₁T₂N₃) from which a clonal derivative was isolated (JWY101). The second line was exposed to H₂O₂ and allowed to recover overnight in YMM. From this population (H₁H₂), a clonal derivative was isolated (JWY102).