Figure 1.—

Colony morphologies of mutants with magellan-4 insertions that abolish S motility in the mglA-8 masK-815 genetic background. Colonies formed by strains (A) DK1622 (wild type), (B) DK4135 (mglA-8), (C), MxH1104 (mglA-8 masK-815), (D) MxH1189 (mglA-8 masK-815 mis-189), (E) MxH1195 (mglA-8 masK-815 mis-195), and (F) MxH1195 (mglA-8 masK-815 mis-198) are shown. The mis-189, mis-195, and mis-189 alleles are magellan-4 insertions in the MXAN_7103, MXAN_6679, and MXAN_4710 genes, respectively (Table 1), predicted to encode a homolog of the CheR methyltransferase named SgnI, a large protein with YD repeat motifs named SgnH and an enzyme that is involved in the LPS biosynthesis pathway named SgnG. Photographs were taken using a Nikon FXA microscope, and are shown at 15× magnification.