TABLE 1.
Coordinates | Locus | Insertion | Straina | Strainb | Gene | Isoallelesc |
---|---|---|---|---|---|---|
5901872–5901873 | MXAN_4707 | mis-185 | MxH1185 | sgmU; rfaF | mis-55 | |
5903725–5903726 | MXAN_4710 | mis-198 | MxH1198 | MxH1298 | sgnG; rfaE | |
7149933–7149934 | MXAN_5774 | mis-178 | MxH1178 | pilO | mis-78, mis-79 | |
7151612–7151613 | MXAN_5776 | mis-168 | MxH1168 | pilM | ||
7151612–7151613 | MXAN_5776 | mis-182 | MxH1182 | pilM | ||
8154610–8154611 | MXAN_6627 | mis-181 | MxH1181 | sgnC | mis-52 | |
8200360–8200361 | MXAN_6671 | mis-196 | MxH1196 | sglK | mis-45 | |
8208665–8208666 | MXAN_6679 | mis-195 | MxH1195 | MxH1295 | sgnH | |
8671663–8671664 | MXAN_7103 | mis-189 | MxH1189 | MxH1289 | sgnI | |
9064019–9064020 | MXAN_7441 | mis-190 | MxH1190 | epsH | ||
9072529–9072530 | MXAN_7448 | mis-179 | MxH1179 | epsD | ||
9072529–9072530 | MXAN_7448 | mis-180 | MxH1180 | epsD |
The 12 insertions of magellan-4 in the M. xanthus genome described in this report are listed in order of their sites within the genome sequence, which can be found at: http://www.tigr.org/tigr-scripts/CMR2/GenomePage3.spl?database=gmx. Given are the gene numbers in which the insertions are situated, the allele numbers of the insertions, the strain numbers of the derivatives of strain MxH1104 with each insertion, and the gene names. The new genes identified by magellan-4 insertions in this study, MXAN_4710, MXAN_6679, and MXAN_7103, have been designated sgnG, sgnH, and sgnI, respectively.
Strain name of the original isolate containing the mariner insertion in M. xanthus MxH1104 (mglA8 masK-815).
Strain name of the corresponding mariner insertion in the wild-type M. xanthus (DK1622) background.
The allele numbers of insertions that were also isolated in an ΔaglU are listed as isoalleles (Youderian and Hartzell 2005).