TABLE 2.
Growth of strains containing mutations throughout the length of Tra1
| Viabilityb
|
|||
|---|---|---|---|
| Mutationa | Reason for analysis | 30° | 37° |
| DSP171 | Potential phosphorylation | Yes | Yes |
| EFS184 | Potential phosphorylation | Yes | Yes |
| KEL345 | Conservedc | Yes | Yes |
| VLL837 | LXXLL sequence | Yes | Yes |
| VRL867 | Conserved | Yes | Yes |
| KKK1686 | Found in chromatin associated Sth1 | Yes | Yes |
| GLW2677 | Conserved in yeastd | Yes | Yes |
| GYH2939 | Conserved in yeast | Yes | Yes |
| TLP2972 | Conserved in yeast | Yes | Yes |
| HFQ3168 | Conserved in FAT domaine | Yes | Yes |
| EER3416 | Conserved | Yes | Reduced |
| PFR3621 | Conserved in PI3K domain | No | No |
a
Alleles were constructed by placing a NotI linker encoding a triple-alanine tag in place of the indicated three amino acids. The number refers to the amino acid position of the first of the three amino acids.
b
Growth on YPD media at 30° or 37°.
c
Conserved indicates identity in S. pombe and human Tra1 molecules.
d
Sequence similarity or identity in yeast homologs.
e