TABLE 3.
Phenotypes of tra1 mutations
| Mutationa | 30°b | 37°c | Phosd | Inoe | EtOHf | VP16g | Benh |
|---|---|---|---|---|---|---|---|
| K3571 | ++++ | ++++ | ++++ | ++++ | ++++ | — | ++++ |
| P3655 | ++++ | ++++ | ++++ | ++++ | +++ | — | ++++ |
| P3408 | +++ | + | + | +++ | + | — | ++ |
| SRR3413 | ++ | — | + | ++ | — | — | + |
| EER3416 | ++++ | ++ | ++++ | ++++ | ++++ | — | ++++ |
| S3463 | ++++ | + | ++ | +++ | + | — | +++ |
| F3443 | + | — | — | — | — | — | — |
| P3446 | ++++ | ++++ | ++++ | ++++ | +++ | +/− | ++++ |
| DKL3491 | ++++ | ++++ | ++++ | ++++ | ++++ | +/− | ++++ |
| P3612 | ++++ | ++++ | ++++ | ++++ | +++ | +/− | ++++ |
| N3617 | ++++ | ++++ | ++++ | ++++ | +++ | +/− | ++++ |
The indicated mutations were engineered into TRA1SB, which contains a BamHI restriction site that results in N3580A and a silent SalI restriction site that converts nucleotides A9714G and T9717G. All amino acid changes were to alanine.
Alleles were transformed into CY1021 and tested for viability by growth on mimimal plates containing 5-FOA. Growth at 30° was determined on YPD. Comparisons were made to a strain containing TRA1SB.
Growth at 37° on YPD plates.
Growth on YPD plates deficient for phosphate.
Growth on complete minimal media lacking inositol.
Growth on YPD plates containing 6% ethanol.
Strains were transformed with padhGV16-gal4-VP16 (Berger et al. 1992) and growth was examined on minimal media lacking leucine.
Growth on synthetic complete media containing 20 μg/ml benomyl.