Figure 2.

Effect of the inhibition of COX-1 with SC-560 on PGE₂ production and COX-2 upregulation in ATRA-treated MC. Cells were preincubated for 1 h with 100 nM SC-560 and then for 24 h with 10 μM ATRA. (a) PGE₂ production, determined in triplicate in four separate experiments. ^*P<0.01 vs control, ^**P<0.05 vs control and ATRA groups. (b) Expression of COX-2 protein (Western blot analysis). Equal protein loading was confirmed by probing with an anti-α-actin antibody. Normalized density ratio of COX-2 over α-actin is indicated for each band. The photograph represents at least three repeated experiments.