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. 2006 Aug 7;149(2):215–225. doi: 10.1038/sj.bjp.0706842

Figure 3.

Figure 3

ATRA-induced increase of COX-2 protein expression is inhibited by actinomycin D and cycloheximide and not owing to increased stability of COX-2 protein. Expression of COX-2 protein after 24 h incubation with ATRA vehicle in control conditions or with 10 μM ATRA was analysed by Western blot in MC. (a) Preincubated for 1 h in control conditions or with 2 μg/ml actinomycin D or (b) preincubated for 1 h in control conditions or with 10 μM cycloheximide or (c) incubated for 0–24 h with 10 μM cycloheximide (Chx) to analyse the stability of COX-2 protein. Equal protein loading was confirmed by probing with an anti-α-actin antibody. Normalized density ratio of COX-2 over α-actin is indicated for each band. Each photograph represents at least three repeated experiments. The graph in panel c represents the COX-2 protein levels normalized to α-actin. Results from the densitometric analysis were expressed as arbitrary values and plotted relative to the zero time point. The protein stability was similar between control and ATRA-treated cells, the half-life being <9 h in both groups.