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. 2006 Aug 7;149(2):215–225. doi: 10.1038/sj.bjp.0706842

Figure 5.

Figure 5

Effect of pharmacological modulation of retinoic acid receptors (RARs) in MC. Western blot analysis of (a,b) the expression of COX-2 protein after 24 h incubation with vehicle or with 10 μM ATRA in cells pretreated for 1 h with (a) RAR pan-antagonist LE540 (2.5 μM) or (b) RAR pan-antagonist AGN193109 (2.5 μM). (c) The expression of RARβ protein after 24 h incubation with vehicle or with 10 μM ATRA in MC pretreated for 1 h with either RAR pan-antagonist LE540 (2.5 μM). (d) The expression of COX-2 protein after 24 h incubation with the RAR pan-agonist TTNPB. Equal protein loading was confirmed by probing with an anti-α-actin antibody. Normalized density ratio of COX-2 or RARβ over α-actin is indicated for each band. The photograph represents at least three repeated experiments.