Figure 1.

RNase H cleavage pattern of oligos on target sequence. The RNA was labeled at the 3′ end and incubated with the oligos as described in Materials and Methods. The samples were analyzed by gel electrophoresis. Arrows indicate the approximate locations of the major (large arrow) and minor (small arrow) excission sites of the RNA by RNase H in the presence of oligos 1, 9, 11, and 14–16. The box in the oligo represents the position of the modified region.