Figure 4.
Original traces showing BMSA and bimakalim (BM) as openers of the Kir6.2/SUR2B channel in inside-out patches. Patches were exposed to symmetrical high-K+ solution at 22°C and clamped at −50 mV. The black bars indicate the application of MgATP (1 mM) and the dotted lines the zero current level. Initial intermittent application of MgATP defined the 100% level of the ATP-inhibited current, IKATP. (a) BMSA (100 μM) induced an inward current that was abolished by glibenclamide (GBC, 1 μM). (b) Effect of bimakalim (1 and 50 μM). For evaluation of the currents see Table 2.