Table 2.
Channel opening effect of BMSA and bimakalim
| Condition | Opener | IKCO (% IKATP or pA)a | t1/2 (min) |
|---|---|---|---|
| i/o (22°C) | BMSA | 72±11% | 0.4±0.1 |
| BM | 57±7% | 1.8±0.1 | |
| BM (50 μM) | 81±4% | 0.10±0.02 | |
| wc (22°C) | BMSA | 0 | — |
| BM | 440±220b pA | 2.1±0.2 | |
| wc (37°C) | BMSA | 0 | — |
| BM | 570±180b pA | 0.4±0.1 | |
| On cell (37°C) | BMSA | 0 | — |
| BM | 490±130 pA | 1.4±0.1 |
Abbreviations: BM, bimakalim; BMSA, descyano-bimakalim-6-sulphonic acid i/o, inside-out; wc, whole- cell, on cell, cell-attached; IKCO denotes the opener-induced current.
Experiments were performed as shown in Figures 4 and 5. BMSA concentration was 100 μM and BM 1 μM if not stated otherwise. Data are means±s.e.m. of 4–7 experiments.
a
In the experiments using the inside-out configuration, IKCO was expressed as % of the current in the absence of MgATP (% IKATP); in the other experiments, as pA.
b
Basal (dialysis-induced) currents of 11 pA (22°C, n=4) or 110±21 pA (37°C, n=6) were subtracted.