Interactions between obestatin and ghrelin on EFS-evoked, nerve-mediated contractions in rat isolated forestomach. (a) Representative trace of ghrelin 0.1 μM-induced increase of EFS-evoked contractions. The bars indicate the periods of EFS, as described in Methods. (b) Effects of obestatin, 0.01 nM–1 μM, on ghrelin 0.1 μM-induced potentiation of EFS-evoked, nerve-mediated contractions in rat isolated forestomach compared to vehicle controls; obestatin administered 1 min before 15 min application of ghrelin; n=8–18, *P<0.05.