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Agonist-evoked cAMP accumulation in HEK293 cells expressing 5-HT4 receptors at a low (clone 1) and high (clone 2) receptor density, respectively. Data points (mean and s.e.m. (vertical lines)) from at least three separate experiments were used to generate curves for 5-HT (a), tegaserod (b), cisapride (c), mosapride (d) and metoclopramide (e) that were normalized to the maximum 5-HT-evoked response.
