Table 2.

Compound I is more cytotoxic than MJ in a range of cancer cell lines

Cell line	IC50 (mM)
	MJ	Compound I
B16-F10	2.6±0.1	0.042±0.002*
MCF7	1.5±0.06	0.015±0.00057*
MIA PaCa-2	1.4±0.09	0.009±0.0011*
D122	1.8±0.18	0.05±0.0074*
PBL	>3	0.25±0.015*

^aAbbreviations: MJ, methyl jasmonate; PBL, peripheral blood lymphocytes.

B16-F10, MCF7, MIA PaCa-2 and D122 cells (at 5 × 10³ per well) were seeded in 96-well plates. Normal lymphocytes (PBL) were pre-treated with PHA (0.8 μg ml⁻¹) and TPA (5 ng ml⁻¹) for 24 h, to induce cell proliferation, and then seeded in 96-well plates (at 1.2 × 10⁵ per well). MJ and Compound I were added at concentrations of 0.001–3 mM for 24 h at 37°C. Cell viability was determined by the XTT cell proliferation assay. IC₅₀ represents the concentration of an MJ derivative that induces death in 50% of the cells (mean±s.d, n=3). P<0.05 comparing MJ and compound I in all cancer cells lines by ANOVA

^*P<0.05, comparing pairwise MJ with compound I in each cancer cell line by the Tukey–Kramer test.