Figure 7.

Effect of the histone deacetylase inhibitor trichostatin A on the FTY720-mediated inhibition of sPLA₂ expression in mesangial cells. Mesangial cells were stimulated for 24 h with either vehicle (−), interleukin-1β (IL-1β, 1 nM), or IL-1β plus FTY720 (3 μM) in the presence of the indicated concentrations of trichostatin A (in nM; pretreated for 30 min before stimulation) as indicated. Thereafter, supernatants were taken for protein precipitation followed by protein separation by SDS-PAGE, transfer to nitrocellulose and Western blot analysis using a specific monoclonal antibody against rat IIA-sPLA₂ antibody at a dilution of 1 : 60. Data are representative of three independent experiments giving similar results.