Figure 5.

Time-course study of sAPPα secretion in the hippocampus by prucalopride in adult APP/V717I transgenic mice. The mice were treated with a single dose of prucalopride (5 mg kg⁻¹, s.c.) or saline (5 ml kg⁻¹) (clear bar). Brain sAPPα and β-actin levels (used as internal control) were measured 30, 90 and 240 min after the injection. In (a) a representative immunoblot illustrating the effects of time on sAPPα levels is shown. In (b), the mean densitometric values (±s.e.m.) of sAPPα levels, relative to β-actin (used as internal control), are expressed as percentages of values in untreated control mice. One-way ANOVA, F(3,11)=5.414. ^*P<0.05 and ^**P<0.01 compared with saline-treated mice (t=0); least significant difference test; n=4 mice per group.