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. 2007 Jan 2;150(3):271–280. doi: 10.1038/sj.bjp.0706983

Figure 5.

Figure 5

Effect of MEK, p38-MAPK and PKC inhibitors on ATP-induced SK-1 activation in renal mesangial cells. Quiescent mesangial cells were preincubated for 20 min with the indicated inhibitors (Ro 31-8220 at 10 μM; U0126 at 20 μM; SB203580 at 10 μM) before stimulation for 6 h with either vehicle (Co), ATP or UTP (each 100 μM). Thereafter, cell lysates containing 50 μg of protein were taken for an in vitro SK-1 activity assay as described in the Methods section. Data are expressed as % of control values and are means with vertical lines showing s.d. (n=4) *P<0.05, **P<0.01 considered statistically significant when compared to the unstimulated control values; #P<0.05, ##P<0.01 considered statistically significant when compared to the ATP- or UTP-stimulated values, respectively; NS, not significant.