Figure 7.

Effect of S1P and extracellular nucleotides on mesangial cell migration. Quiescent mesangial cells (1 × 10⁵) were used for a migration assay employing a modified Boyden chamber assay system as described in the Methods section. Cells were treated for 24 h with either vehicle (control), or with 1 μM of S1P (a), 100 μM of ATP (b) and 100 μM of UTP (b), at the time periods indicated in h, or cells were treated for 24 h with the indicated concentrations (in μM) of ATP (c) or UTP (c). Data are expressed as % of control values and are means with vertical lines showing s.d. (n=3–6), ^*P<0.05, ^#P<0.05, ^**P<0.01, ^##P<0.01, ^***P<0.001, ^###P<0.001 considered statistically significant compared to the corresponding control values.