Figure 2.

Effect of budesonide on P-gp protein expression. LS180 and Caco-2 cells were incubated for 72 h with 25 μM budesonide or vehicle. Rifampicin was used as a positive control for MDR1 induction in LS180 cells. P-gp protein and β-actin were determined by Western blot analysis. β-Actin was used as loading control.